ICSI

The quality of the oocytes is very variable from one mare to another and tends to decline with the aging of the donor, bringing lower success rates compared to younger mares.

The number of oocytes can also be a limiting factor. Some mares have multiple follicles increasing the chances of obtaining oocytes.

Other mares usually have few and, in this case, we have smaller chances.

On average, it takes 3 cycles of aspiration to establish one pregnancy.

But, this is an average, as some mares (30%) generate multiple pregnancies in a single OPU, while others, with few oocytes or low viability semen, will have a greater challenge to produce an embryo and pregnancy.

• Contention stock in a covered place;
• Electricity point near the stock;
• Table or bench to support the OPU equipment.
• Water point near the stock, for cleaning the mares;
• At least one person from the stud/center, who is responsible for accompanying the mare in the stock throughout the procedure;
• Laboratory/room for oocyte selection: bench or table with energy and water point. This location must be a room/laboratory that will be closed practically during the entire procedure. One day before the departure of our team, this place must be cleaned with only water, neutral detergent, 70% alcohol, and a clean cloth. Do not use chemical products that may leave odor or residues, such as creolin and bleach, when cleaning.

Yes, but the laboratory will use more than one stallion for the same donor only when she has at least 8 mature oocytes.

We prefer to use one stallion per mare per aspiration.

The vitrification protocol currently used in our laboratory has produced the same pregnancy rate as that of fresh embryos. The advantage of vitrification is that these embryos can be thawed at a time when the supply of quality recipients is greater, contributing significantly to the result.